Spectrophotometric Method for the Determination of Cefotaxime Sodiumand Cefoperazone Sodium in Pure and Pharmaceutical Dosage Forms
Rania Adel Sayed *
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, El- Gamah street, Zagazig,44519, Egypt
Wafaa Sayed Hassan
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, El- Gamah street, Zagazig,44519, Egypt
Magda Yossif El- Mammli
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, El- Gamah street, Zagazig,44519, Egypt
Abdalla Shalaby
Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, El- Gamah street, Zagazig,44519, Egypt
*Author to whom correspondence should be addressed.
Abstract
Accuratespectrophotometric method was developed for the estimation of cefotaxime sodium (I) andcefoperazone sodium (II) in both pure and pharmaceutical dosage forms. The method is based on the reaction of the amino groups of the cited drugs with ninhydrin reagent producing a colored product which absorbs maximally at 568 nm. Beer's law was obeyed in the concentration range of (10-90) and (60-350) μg.mL-1 for drug (I) and (II) respectively .The correlation coefficient (r2),molar absorptivity (ε), sandell sensitivity, detection (LOD) and quantitation limits (LOQ) for the studied drugs were calculated. The proposed method was successfullyapplied for the determination of certain pharmaceutical dosage forms containing the studied drugs.
Keywords: Cefotaxime sodium, cefoperazone sodium, spectrophotometric, condensation, ninhydrin