Potential Effect of Vitamin C and Curcumin on Oxidative Stress and Skin Lesion Induced by Dermal Intoxication with Cypermethrin
Manal E. A. Elhalwagy *
Biochemistry Department, King Abdulaziz University, Faculty of Science for Girl's, P.O.Box 51459, Jeddah- 21453, Saudi Arabia and Mammalian and Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center, Giza 12618, Egypt
A. A. Nahas
Mammalian and Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center, Giza 12618, Egypt
Reem M. Ziada
Mammalian and Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center, Giza 12618, Egypt
Hoda E. A. Farid
Department of Biochemistry, Faculty of Agriculture, Menofia University, Egypt
*Author to whom correspondence should be addressed.
Abstract
Aim: Dermal exposure to pyrethroids produces adverse health effect on different body systems. This study examined the effect of simultaneous oral supplementation of curcumin and vitamin C on systemic and skin injuries induced by dermal intoxication with cypermethrin.
Experimental Design: Four groups of rats treated for 28 days, -ve control, +ve control (curcumin 200 mg/Kg) and vitamin C (100 mg/Kg), Pesticide (cypermethrin (1/25 LD50 = 200 mg/Kg) on shaved skin of rats and pesticide and antioxidant (rats dermally intoxicated with cypermethrin (1/25 LD50 = 200 mg/Kg) and supplemented orally with (curcumin 200 mg/Kg) and vitamin C (100 mg/Kg).
Results: Dermal intoxicated rats with cypermethrin (1/25 LD50 = 200 mg/Kg) showed Inhibition in ATPase enzyme in serum, liver and brain associated with Induction of oxidative stress biomarkers malodialdehyde (MDA) and protein carbonyl in serum, liver but not in brain, concomitant with significant reduction in defense system serum SH-protein and reduced glutathione in liver and brain. As well as significant decrease in antioxidant enzymes activities superoxide dismutase (SOD), glutathione -S- transferees (GST) and catalase in serum, liver and brain. Penetration of cypermethrin through skin of treated rats induced necrosis and scales formation in the superficial layer of the epidermis of animals associated with inflammatory cells.
Conclusion: Simultaneous Supplementation with curcumin and vitamin C orally improved the alteration in the above mentioned parameters. However it failed to counteract the injures in the skin. Finally dermal exposure to pyrethroids threat the health of exposed subjects and needs long time in retrieve their health.
Keywords: Dermal toxicity, Pyrethroids, oxidative stress, liver, brain, skin