Screening of Alpha Amylase Inhibitory Activity and Antioxidant Activity of Selected Sri Lankan Medicinal Plants and Development of Herbal Tea
Chemical Science International Journal,
Aims: Herbal medicines are widely used because of its health benefits and minimum side effects. There are many underutilized medicinal herbs in Sri Lanka which are not subjected to any scientific study to prove their health benefits. The present study attempted to identify the health benefits and possibility to develop herbal teas from selected herbs; Solanum trilobatum, Ocimum tenuiflorum, Cardiospermum halicacabum, Acalypha indica and Plectranthus amboinicus.
Study Design: Complete Randomized Design was employed.
Place and Duration of Study: The study was carried out in Agricultural Chemistry Laboratory of Uva Wellassa University, Badulla, Sri Lanka From November 2018 to March 2019.
Methodology: Leaf samples of these herbs were oven dried for 12 hours at 50 . Infusions were prepared by boiling varying amounts of leaf samples from 1g to 5g in 100 ml of distilled water and tested for antioxidant activity with DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay and α-amylase inhibition activity using Dinitrosalicylic acid method. Sensory evaluation was conducted for the same infusions with ISO-3103 specifications to select the best amounts of samples for consumption. The selected amounts were subjected to chemical analysis.
Results: The best antioxidant activity and α-amylase inhibition activity were observed in C.halicacabum with 7.71 mg ml-1 and 45.51 mg ml-1 IC50 values respectively. Results confirmed that all leaf infusions contain Anthocyanin (4.20-0.11mg dm-3), Polyphenols (120.49-32.93 mg dm-3 and Free Sugar (262.95-24.67mg dm-3).
Conclusion: Selected herbs show positive responses towards selected biochemical properties and there is a very good potential to develop herbal teas with interesting health benefits with high customer attraction. C. halicacabum shows the best potential among selected herbs. However, it is suggested to carry out further studies to confirm these results and find out any adverse effects of overconsumption.
- α-Amylase inhibition
- free sugar
- siddha medicine.
How to Cite
Chan EWC, Lim YY, Chong KL, Tan JBL Wong SK. Antioxidant properties of tropical and temperate herbal teas. Journal of Food Composition and Analysis. 2010; 23(2):185-189.
Kumar RS, Raja NK, Vijay M, Raja CSG. Anti-oxidant, anti-diabetic, antimicrobial and hemolytic activity of Solanum torvum and Solanum trilobatum. Journal of Pharmaceutical Sciences and Research. 2016;8(8):725.
Mazid M, Khan TA, Mohammad F. Medicinal plants of rural India: A review of use by Indian folks. Indo Global Journal of Pharmaceutical Sciences. 2012; 2(3): 28630.
Chandrasekara A, Shahidi F. Herbal beverages: Bioactive compounds and their role in disease risk reduction-A review. Journal of Traditional and Complementary Medicine. 2018;8(4): 451458.
Farzaneh V Carvalho IS. A review of the health benefit potentials of herbal plant infusions and their mechanism of actions. Industrial Crops and Products. 2015;65:247-258.
Mohanta TK, Patra JK, Rath SK, Pal DK, Thatoi HN. Evaluation of antimicrobial activity and phytochemical screening of oils and nuts of Semicarpus anacardium Lf. Scientific Research and Essays. 2007; 2(1):486-490.
Kamilogo S, Toydemir G, Boyacioglu D, Esra C. Health perspectives on herbal tea infusions. Phytotherapeties. 2016;43.
Atoui AK, Mansouri A, Boskou G, Kefalas P. Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chemistry. 2005;89(1):27-36.
Silva CG, Herdeiro RS, Mathias CJ, Panek AD, Silveira CS, Rodrigues VP, Renno MN, Falcão DQ, Cerqueira DM, Minto ABM, Noguera FLP, Quaresma CH, Silva JFM, Menezes FS, Eleutherio ECA. Evaluation of antioxidant activity of Brazilian plants. Pharmacol Res. 2005;52: 229–233.
Nickavar B, Abolhasani L, Izadpanah H . α -amylase inhibitory activities of six Salvia species. Iran J Pharm Res. 2008;7:297–303.
ISO. Content of total polyphenol in tea. Colorimetric method using Folin Ciocalteu. 2005;14502-1.
Sutharut J, Sudarat J. Total anthocyanin content and antioxidant activity of germinated colored rice. Int Food Res J. 2012;19:215–221.
Miller GL. 1959Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry. 2012;31(3): 426-428.
Chirag JP, Tyagi S, Halligudi N, Yadav J, Pathak S, Singh SP, Pandey A, Kamboj DS, Shankar P. Antioxidant activity of herbal plants: A recent review. J Drug Deliv Ther. 2013;1:1-8.
Kumaran A, Joel Karunakaran R. Antioxidant Activities of the Methanol Extract of Cardiospermum halicacabum. Pharmaceutical Biology. 2006;44(2):146-151.
Teklani PWNN, Perera BGK. The important biological activities and phytochemistry of Acalyphaindica. International Journal of Research in Pharmacy & Science. 2016;6(1):30-35.
Fabiola, Sumathy VJH. A comparative study on the phytochemicals, antioxidant, antidiabetic and antimicrobial activity of the leaves of solanum nigrum and Solanum trilobatum. European Journal of Pharmaceutical and Medical Research. 2017;4(9):331-335.
Das MP, Yasmine Y, Vennila Devi P. Evaluation of qualitative phytochemical constituents and in vitro anti-diabetic activity of Cardiospermum halicacabum L. International Journal of Pharma and Bio Sciences. 2015;6(3):711-719.
Cobb DT ed.2014.Polyphenols: Food Sources, Bioactive Properties and Antioxidant Effects. Nova Publishers.
Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Kong M, Li L, Zhang Q, Liu Y, Chen H. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules. 2016;21(10):1374.
Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food & Nutrition Research, 2017; 61(1):1361779.
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