Main Article Content
Prunus africana belongs to the Rosaceae family. It is a geographically wide spread tree to forest habitats of the African continent. P. africana is one of the most popular plants in traditional medicine for treating various ailments. It is mainly used to treat benign prostate hyperplasia (BHP). The study was aimed to evaluate the anti-bacterial and anti-oxidant activities of bark of root extract from P. africana. The air dried and powdered plant material (200 g) was first soaked with 500 mL of n-hexane for 72 hours and yielded 2.5 g of n-hexane extract. Residue was soaked with 500 mL of ethyl acetate for 72 hours and afforded 3.7 g of ethyl acetate extract. Finally, residue was soaked with 400 mL of methanol and yielded 14.3 g of methanol extract. The methanol extract showed inhibition zones of 18 and 14 mm against Escherichia coli and Staphylococcus aureus, respectively. The extracts also showed encourage results of DPPH radical scavenging activity at various concentrations. The methanol extract of P. africana of root showed promising activity against E. coli, ATCC25922 and S.aureus, ATCC25922. Anti-oxidant activities also were shown prospective result, selectively at lowest concentration and lowest absorbance. This means the result of the study was confirmed that the lowest concentration of 4 mg/mL and absorbance of 0.112 the scavenging activity was 87.9%, while at the highest concentration of 128 mg/mL and absorbance of 0.172 the scavenging activity was 81.5%.
Taxonomy. Economic importance and genomics of Rosaceae; 2019.
Available:www.hort.purdue.edu /newcrop/janick.../rosaceae.pdf-United States, downloaded in Mar.10/2019.
Zhang X. The role of WHO in traditional medicine worldwide: Drug Information Journal. 1999;33:321–326.
Yibralign Z. Phytochemical investigation on the stem bark of Croton macrostachyus, M.Sc. Thesis, Addis Ababa University, Ethiopia. 2007;1-6.
Ochwang’i DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. Medicinal plants used in treatment and management of cancer in Kakamega County, Kenya. J. Ethnopharma. 2014; 151(3):1040–1055.
Steenkamp V. Phytomedicines for the prostate. Fitoterapia. 2003;74(6):545–552.
Ting H, Deep G, Agarwal C, Agarwal R. Te strategies to control prostate cancer by chemoprevention approaches. Mutation Research. 2014;760:1–15.
Gilligan T, Kantoﬀ PW. Chemotherapy for prostate cancer. Urology. 2002;60(3):94–100.
Landis-Piwowar KR, Iyer NR. Cancer chemoprevention: Current state of the art. Cancer Growth and Metastasis. 2014;7: 19–25.
Bhanot A, Sharma R, Noolvi MN. Natural sources as potential anti-cancer agents: A review. Int. J. Phytomed. 2011;3(1):9–26.
Cragg GM, Grothaus PG, Newman DJ. Impact of natural products on developing new anti-cancer agents. Chemical Reviews. 2009;109(7):3012–3043.
Kadu CAC, Parich A, Schueler S. Bioactive constituents in Prunus africana: Geographical variation throughout Africa and associations with environmental and genetic parameters. Phytochemistry. 2012; 83:70–78.
Grace OM, Prendergast HDV, Jager AK, Van Staden J. Bark medicines used in traditional healthcare in KwaZuluNatal, South Africa: An inventory. South African Journal of Botany. 2003;69(3):301–363.
Nyamai DW, Arika WM, Rachuonyo HO, Wambani JR, Ngugi MP. Herbal management of benign prostatic hyperplasia. J. Canc. Sci. Terap. 2016; 8(5):130–134.
Jena AK, Vasisht K, Sharma N, Kaur R, Dhingra MS, Karan M. Amelioration of testosterone induced benign prostatic hyperplasia by Prunus species. J. Ethnopharmac. 2016;190:33–45.
Nyamai DW, Mawia AM, Wanbua FK, Njoroge A, Matheri F. Phytochemical profle of Prunus africana stem bark from Kenya. J. Pharmaco. Nat. Prod. 2015;1:110.
Ngule MC, Ndiku MH and Ramesh F. Chemical constituents screening and in vitro antibacterial assessment of Prunus africana bark hydromethanolic extract. J. Nat. Sci. Rese. 2014;4(16):85–90.
Chrispus M, Mueni N, Ndiku H, Ramesh F. Chemical constituents screening and in vitro antibacterial assessment of Prunus africana bark hydromethanolic extract: J. Nat. Sci. Rese. 2014;4(16):2224-3186.
Freeman D. Antibiotic resistance patterns of pseudomonas aeruginosa and Escherichia coli isolates from three hospitals in Kumasi, M.Sc. Thesis, Kwame Nkrumah University, Ghana. 2011;1-95.
Huang Y. Inactivation of Escherichia coli O157:h7 on baby spinach by aqueous and aerosolized antimicrobials, M.Sc. Thesis, University of Delaware, Newark, DE 19716, United States. 2011;1-111.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol. 1995;28:25-30.
Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos Santos TC, Coube CS. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001;15:127-13.