Isolation and Characterization of Zygadenine- N- ribofuranoside from the Leaves of Nymphaea alba Lily
Peter Chidi Njoku *
Department of Chemistry, Alvan Ikoku Federal University of Education, P.M.B. 1033, Owerri, Imo State, Nigeria
Johnbull O. Echeme
Department of Chemistry, Michael Okpara University of Agriculture, Umudike P.M.B. 7267, Umuahia, Abia State, Nigeria
Rosemary I. Uchegbu
Department of Chemistry, Alvan Ikoku Federal University of Education, P.M.B. 1033, Owerri, Imo State, Nigeria
Emeka C. Ogoko
Department of Chemistry, National Open University of Nigeria, P.M.B.80067, Lagos, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Aims: To isolate and characterize a new secondary metabolite, zygadenine-N-ribofuranoside derivative from the leaves of water lily (Nymphaeae alba L.).
Methodology: The ethanol extract of the dried leaves was partitioned between chloroform and water (1:1), then the chloroform fraction was further partitioned between petroleum ether (60-80°C) and aqueous methanol (1:1). Column chromatography of the chloroform fraction over silica gel (200 mesh) by a gradient elution technique from petroleum ether to chloroform, followed by methanol was carried out.
Results: A yellow solid 0.22 g was isolated which was characterized by employing IR, 1D and 2D NMR techniques spanning 1H, 13C, 1H-1H COSY, 1H-13C COSY, HMBC and TOF ESI-mass spectrometry.
Conclusion: Zygadenine-N-ribofuranoside derivative isolated, gave m/z 622.4631, corresponding to molecular formula C33H54O9N2.
Keywords: Nymphaea alba, ribofuranoside, secondary metabolite, zygadenine