Synthesis, Characterization, DNA Binding, Antibacterial Activity and DFT Calculation of Ru(II) Complexes of Hydroxamic Acids and 1,10-Phenanthroline
Rakesh K. Sahu
Department of Chemistry, National Institute of Technology Raipur, Chhattisgarh, India
Fahmida Khan *
Department of Chemistry, National Institute of Technology Raipur, Chhattisgarh, India
*Author to whom correspondence should be addressed.
Abstract
New Ruthenium (II) complexes of mixed ligands, hydroxamic acid (L1 = AHA (acetohydroxamic acid) and L2 = BHA (benzohydroxamic acid)) and 1, 10-phenanthroline (phen) were synthesized, characterized by FT-infra red spectrophotometer, UV-visible spectrometer, ESI-mass spectrometer and elemental analysis. In the complexes, [Ru(phen)2L] (L = AHA, BHA), the metal ion is bind with 6 atoms, 2 O-atoms from hydroxamic acids (AHA & BHA) and 4 N-atoms from 1, 10-phenanthroline to form octahedral Ruthenium (II) complexes. The binding of these complexes with CT-DNA has been studied by UV-visible spectrophotometer. The DNA-binding coefficients for complexes [Ru(phen)2AHA] and [Ru(phen)2BHA] are 2.29 x 106 M-1 and 3.30 x 106 M-1 respectively. Detailed study indicates that these complexes bind with DNA by intercalation binding. Also studied microbial activity of these complexes against Gram positive and Gram negative bacteria. The structure of metal complexes were optimized and calculated by the Gaussian 09 (G09) software.
Keywords: DNA binding, hydroxamic acids, Ruthenium(II) complexes, 1, 10-phenanthroline, antibacterial activity, DFT