Corrosion Resistance and Antimicrobial Activity of Extra- and Intracellular Fe(II) Nanoparticles Biosynthesized Via Aspergillus foetidus ATCC 14916
Nagwa M. Sidkey
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University (Girls Branch), Youssif Abbas St., Nasr City, Cairo, Egypt
Yasser M. Moustafa
Egyptian Petroleum Research Institute, Zaker Hussein St., Nasr City, Cairo, Egypt
Rawhia A. Arafa
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University (Girls Branch), Youssif Abbas St., Nasr City, Cairo, Egypt
Rania E. Morsi
Egyptian Petroleum Research Institute, Zaker Hussein St., Nasr City, Cairo, Egypt
Mai M. Elhateir *
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University (Girls Branch), Youssif Abbas St., Nasr City, Cairo, Egypt
*Author to whom correspondence should be addressed.
Abstract
This study aims to synthesizing Fe (II) nanoparticles with green synthesis method. For this purpose fungal isolate was chosen from the most potent four isolates and its metallotolerance ability towards Fe (II) was studied. Extracellular and intracellular biosynthesis of the nanoparticles was achieved. The nanoparticles were characterized using atomic absorption spectrophotometer (AAS), dynamic light scattering (DLS) and transmission electron microscope (TEM). The shape of metal nanoparticles mostly was spherical. The size of the particles ranges from 80 nm to 370 nm & from 31.53 nm to 61.94 nm for extra- and intracellular particles respectively. The isolate was identified based on morphological and physiological and genetic characteristics and it was found to be closely related to Aspergillus foetidus strain ATCC 14916 with 99% similarity. The antimicrobial activity of the produced intracellular and extracellular nanoparticles was done. The samples have shown antimicrobial activity against some of the used test organisms with different results and different diameter of the inhibition zones among each other. In addition, the corrosion inhibition efficiency of the extracellular nanoparticles was studied. The inhibition efficiency of the inhibitor by polarization curves exhibited the maximum inhibition efficiency 93.877% at the highest concentration of 907.2 ppm.
Keywords: Metallotolerant, metal nanoparticles, iron, green synthesis, TEM, DLS, AAS, andantimicrobial activity