Selenium Concentration of Iraq Patients Infected with Chronic Hepatitis B Using Different Analytical Techniques
Wahran M. Saod *
Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Hapipah M. Ali
Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Suzy M. Salama
Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
Tahseen A. Zaidan
Department of Chemistry, Faculty of Science, University of Anbar, 55431 Baghdad, 55 Ramadi, Iraq
Abdul Wahab A. Alfaluji
Fallujah Teaching Hospital, Fallujah, Iraq
*Author to whom correspondence should be addressed.
Abstract
Aims: The study aimed to use different analytical techniques for determination Selenium, related to patients with chronic hepatitis (B).
Study Design: Selenium was estimated by using AAS and ICP-MS and The analytical results undergoes comparison for priority of the methods. One hundred sixty patients samples and thirty healthy individuals were investigated in this study. The patients were divided into five groups.
Place and Duration of Study: The study was carried out in Fallujah teaching hospital for the period from 1 May to 3 November at viral hepatitis consultation clinic in the hospital.
Methodology: Selenium was estimated by using AAS and ICP-MS and The analytical results undergoes comparison for priority of the methods. One hundred sixty patients samples and thirty healthy individuals were investigated in this study. The patients were divided into five groups. Count/spleen diameter ratio was also calculated.
Results: In order to determine the appropriate method for measuring selenium, concentrations in the serum, scatter plotting method was employed. The measured readings of AAS and ICP-MS for Selenium, was compared. The results of the study suggest that hydride generation was the preferred method to estimate the concentration of selenium.
Conclusion: According to the results of application of scatter plotting statistical technique, it has been shown Selenium concentration can be determinate by AAS better than ICP-MS. The serum selenium concentrations were significantly lower in chronic hepatitis B patients than healthy individuals.
Keywords: Selenium, ICP-MS, hepatitis B, PCR