Validated Micelle-enhanced Spectrofluorimetric Determination of Serotonin in Human Urine
Abdourahmane Khonté
*
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Khémesse Kital
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Jean Pierre Bakhoum
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Latyr Dione
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Oladele Oyelakin
University of The Gambia, School of Arts and Sciences, Division of Physical and Natural Sciences, Gambia.
Diéne Diégane Thiaré
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Lamine Cisse
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Atanasse Coly
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
Francois Delattre
Laboratoire de Synthèse Organique et Environnement, EA2599, Université du Littoral Côte d’Opale, 145 avenue Maurice Schumann, BP 59140 Dunkerque, France.
Alphonse Tine
Laboratoire de Photochimie et d’Analyse (LPA), Faculté des Sciences et Techniques, Université Cheikh Anta Diop, BP 5005 Dakar, Sénégal.
*Author to whom correspondence should be addressed.
Abstract
A micelle-enhanced spectrofluorimetric method was developed and validated for the determination of serotonin (5-hydroxytryptamine, 5-HT) in aqueous solutions and human urine. Fluorescence responses of 5-HT were evaluated in various surfactant systems, with Brij 700 and sodium dodecyl sulfate (SDS) providing significant signal enhancement. Under optimized conditions (λex 275 nm; λem 340 nm), the method exhibited linearity over 0.1–300 ng mL⁻¹ (R² ≥ 0.996), with limits of detection of 1.7 ng mL⁻¹ in Brij 700 and 2.4 ng mL⁻¹ in SDS. Validation according to ICH Q2(R1) guidelines confirmed good precision (intra-day RSD 1.3–3.6 %, inter-day RSD 3.7–4.3 %), accuracy, selectivity, and reproducibility. Recoveries from fortified urine samples ranged from 89–101 %, demonstrating minimal matrix effects. Compared to conventional chromatographic techniques (HPLC/GC), this micelle-enhanced method is faster, simpler, and cost-effective, making it particularly suitable for routine clinical monitoring of serotonin in human urine.
Keywords: Serotonin (5-HT), micelle-enhanced fluorescence, Brij 700, sodium dodecyl sulfate, urine determination